Ultrasensitive on-column detection of capillary electrophoresis using laser-induced capillary vibration method
نویسندگان
چکیده
منابع مشابه
Ultrasensitive on-column detection of capillary electrophoresis using laser-induced capillary vibration method
Capillary electrophoresis (CE), with its advantage of high resolution power, has attracted much attention. But CE has another advantage of needing only a small sample volume, which is expected to make it a useful tool for ultramicroscale analysis as well. However, conventional absorbance detectors are not sensitive enough because the optical light-path is that of the diameter of a capillary, wh...
متن کاملUltrasensitive on-column laser-induced fluorescence in capillary electrophoresis using multiparameter confocal detection.
We report a simple method to efficiently couple on-column, standard Capillary Electrophoresis with Confocal MultiParameter Fluorescence detection (CE-CMPF) using only commercially available components. A molecular collection of 13% and a concentration limit of detection of 1.5 pM fluorescein are achieved in our instrument by gating the arrival time of individual photons in order to reduce the s...
متن کاملTwo-dimensional capillary electrophoresis: capillary isoelectric focusing and capillary zone electrophoresis with laser-induced fluorescence detection.
CIEF and CZE are coupled with LIF detection to create an ultrasensitive 2-D separation method for proteins. In this method, two capillaries are joined through a buffer-filled interface. Separate power supplies control the potential at the injection end of the first capillary and at the interface; the detector is held at ground potential. Proteins are labeled with the fluorogenic reagent Chromeo...
متن کاملEnd-column detection for capillary zone electrophoresis.
(29) Pszenny, A. A. P.: Harvey, G. R.; Brown, C. J.; Lang, R. F.; Keene, W. (21) Hendbook of ChemistryandPhysics, 56th ed.; CRC Press: Cleveland, OH, 1975. (22) Van Wijk, R. J. Chromatogr. Sci. 1970, 8 , 418-420. (23) Bertsch, W.: Chang, R. C.; Zlatkis, A. J. Chromatogr. Sei. 1974. 72, 175-182. (24) Butler, L. D.; Burke, M. F. J. Chromatogr. Sci. 1976, 74, 117-122. (25) Lang, R. F. Anal. Chem. ...
متن کاملOn-column conductivity detection in capillary-chip electrophoresis.
On-column conductivity detection in capillary-chip electrophoresis was achieved by actively coupling the high electric field with two sensing electrodes connected to the main capillary channel through two side detection channels. The principle of this concept was demonstrated by using a glass chip with a separation channel incorporating two double-Ts. One double-T was used for sample introducti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Analusis
سال: 1998
ISSN: 0365-4877,1286-482X
DOI: 10.1051/analusis:199826050041